Research workflow

Citrus HLB genomic selection workflow: a structured path from DNA sequencing to breeding decisions and experimental validation.

DNA Sequencing

Citrus samples are sequenced to generate genome-wide variation data.

Marker Discovery (SNP Identification)

Key SNPs associated with HLB tolerance are identified from sequencing data.

Genomic Prediction Model

Machine learning and statistical models use SNP data to predict resistance and breeding performance.

Trait Scoring & Accuracy Validation

Each plant is assigned a resistance score and model accuracy is evaluated.

Breeding Decision Dashboard

Top parent plants are selected based on predicted performance, optimizing crossing strategies.

Resistance / Yield Gain Projection

Expected improvement in yield and resistance is estimated.

Experimental Validation

Selected SNPs are validated through laboratory assays.

CRISPR / Targeted Editing (Optional)

Candidate genes may be edited to enhance HLB resistance.

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